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Flow Imaging Cytometry – FlowSight: Cell Biology, Biochemistry

  • Classical biochemical techniques are often employed to measure location and co-location of molecules, tasks ideally suited for Amnis imaging flow cytometry system. Examples include molecular translocation of transcription factors from the cytoplasm to the nucleus, trafficking of molecules to sub-cellular organelles, co-localization of proteins on, in, or between cells, just to name a few. The Amnis imaging flow cytometry’s ability to obtain statistically robust per-cell measurements of probe location and co-localization within highly heterogenous samples provides significant advances that complement and extend traditional biochemical research.
  • Because cell differentiation often involves a dramatic metamorphosis of the stem cell into a committed cell lineage, Amnis imaging flow cytometry is ideally suited for these types of studies. Whether by measuring the location of proteins that help stem cells maintain pluripotency or by quantifying signaling events that lead toward differentiation down a committed cell line, the ability of Amnis imaging flow cytometry to analysis large numbers of cells simplifies identification and classification of rare stem cell populations.

Measurement of embryonic stem cell size

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757755/pdf/main.pdf

Measuring Cell Cycle and Mitotic Index with the FlowSight

http://www.selectscience.net/downloads/articles/7099_EMD_Millipore_13_Flow_Cytometry_Cell_Cycle.pdf

Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors

http://www.pnas.org/content/110/52/21048.short

Hepatectomised patient sera promote hepatocyte differentiation of human-induced pluripotent stem cells

http://www.sciencedirect.com/science/article/pii/S1590865814003363

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