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High Resolution Flow Microscopy – ImageStream X (ISX): Immunology

Cells of the innate and adaptive immune system work in concert to protect the body from pathogenic attack. The many different types of immune cells can be distinguished from one another not only by cell surface markers but also by their diverse cellular responses to different pathogens or stimuli. By combining quantitative image analysis tools with the large sample sizes common to flow cytometry, the ImageStream uniquely enables multiple applications within the field of Immunology, including measurement of nuclear translocation of transcription factors, T:APC interactions and accumulation of proteins at the immune synapse, chemokine-induced shape change, and phagocytosis, just to name a few.

Localization of ADAP to the immunological synapse

NF-kB translocation in T:APC conjugates

Phagocytosis by murine macrophages

HIV-specific translocation of NFAT

http://www.jbc.org/content/288/5/2986.full.pdf+html

Transcription 3 (STAT3) Activation Transducers and Activators of Duration of Receptor and Signal

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