The FlowSight from Amnis offers high performance in a small package. Its innovative design increases signal and minimizes noise to provide unmatched fluorescence sensitivity. Twelve standard detection channels simultaneously produce brightfield, darkfield and up to ten channels of fluorescence imagery of every cell. With these unique capabilities, the FlowSight enables a broad range of applications.




The FlowSight operates like a conventional flow cytometer but also provides imagery of every cell. Powerful and intuitive analysis software seamlessly links quantitative data to imagery:

  • Click on a dot in any plot to see the corresponding cell imagery.
  • Click on a bin in any histogram to view all the cells in that bin.
  • Draw gates on dot plots and view the resulting populations to validate results.

The FlowSight is powerful enough for the core lab but sized and priced for any lab. The system can be factory configured or field upgraded with up to four excitation lasers (405, 488, 561, 642 nm), a 96-well plate AutoSampler, and a powerful quantitative image processing option. Whether in a base configuration or fully-optioned, the FlowSight sets a new standard of value.


Cell Signaling detail Cell Signaling and Molecular Translocation

Measure translocation of transcription factors or membrane proteins, e.g. NF-κB.

Internalization detail Internalization

Measure the internalization of proteins or particles from the cell surface to the interior.

Morphology detail Morphology Analysis

Measure cellular and nuclear morphology to quantify shape change.

Co-Localization detail Co-localization

Measure molecular trafficking to organelles or the co-localization of cell surface or nuclear proteins.

Cell-Cell Interaction detail Cell-Cell Interaction

Measure the accumulation of protein at the immune synapse or the inter-cellular boundary during virus-induced cell fusion.

Cell Cycle and Mitosis detail Cell Cycle and Mitosis

Enhance cell cycle analysis with a mitotic index derived from nuclear morphology.

DNA Damage and Repair detail DNA Damage and Repair

Rapidly and objectively measure the characteristic nuclear staining of DNA repair proteins.

Cell Death and Autophagy detail Cell Death and Autophagy

Quantify DNA fragmentation, the hallmark of apoptosis, and quantify LC3 clustering, the hallmark of autophagy.

Spot Counting detail Spot Counting

Quantify the number of discrete spots in images.

Twelve channel imagery of 3 micron diameter Spherotech 8-peak Rainbow beads.