Cell Sorters

Overview
Cell research is at the forefront of biological study and synthetic biology for the production of many cell-derived products. As cell populations are extremely diverse, it is important to have the ability to physically separate a subset of cells of interest from the rest, this is what cell sorting does. The technology, FACS (fluorescence activated cell sorting) uses laminar flow to create a stream of single cells in single file within a stream of PBS. Each cell is identified using emitted fluorescence from molecular markers and sorted by using an electrical charge. Up to 4 subsets of cells may be either sorted to a separate collection tube, to a 96/384 plate well, the rest of the cells continuing to flow to a waste tank. Thus, pure populations are collected out of a mixture of cells for downstream processing – i.e., cultivation or analysis, such as single-cell DNA/RNA analysis.
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Use Cases
Cell sorting may be used for genetic / epi-genetic studies where DNA or RNA is sequenced to decipher molecular processes, for growing specific cell population of interest for different processes such as gene editing, for separating microorganisms, viruses (in a biological hood) and for sorting algae.
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Unique Benefits
FACS is the only method for sorting sub-populations that utilize multiple markers to distinguish the cells of interest from other cells. FACS is also fast, enabling the sorting of millions of cells in a short time.
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The MA900 is the ‘big-brother’ of the SH800, with the same user-friendliness and flexibility, better sited to immunologists and researchers with complex fluorescent panels – up to 12 fluorescent probes rather than 6 and 2 threshold channels rather than 1. It automatically calibrates and is ready for use within less than 45 min and requires less than a minute of time in front of the system until ready for use. Up to 4 lasers can be installed at 2 contact points, with 12 fluorescent channels excluding FSC and BSC.
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The SH800 is specifically designed for user-friendliness, enabling you to focus on your experiment and its correct execution rather than focussing on ensuring system stability. It automatically calibrates and is ready for use within less than 30min and requires less than a minute of time in front of the system until ready for use. Up to 4 lasers can be installed, with 6 fluorescent channels excluding FSC and BSC.
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The FX500 is one of only 2 systems specifically designed for truly sterile conditions, best suited for applications involving transplants, in vivo studies post-sort, or truly cleaned samples are required.
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Sony Biotechnology's new CGX10 Cell Isolation System sorts cells at high speed and high purity in a closed system. The CGX10's design enables sorting of cells in a manner that is entirely closed off from the outside environment, making it possible to analyze and isolate cells while maintaining a sterile state.
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