Flow Imaging

The ImageStreamX MKII is a revolutionary flow cytometry system that bridges the gap between the speed of traditional flow cytometry and the high-resolution imaging capabilities of microscopy. It achieves this by incorporating three lenses offering 20x, 40x, and 60x magnification, allowing for detailed visualization of samples alongside traditional flow cytometry data. Additionally, the system can image and quantify up to 12 fluorescent probes excited by up to 5 lasers, providing a wealth of multicolor data for comprehensive analysis. Despite its advanced technology, the ImageStreamX remains user-friendly with guided workflows and intuitive software, ensuring researchers can maximize their data output. The system accepts samples in both 1.5 ml tubes and 96-well plates, offering flexibility for various experimental setups.

FlowCam systems characterize microparticles in the wide range of 2-2000um. The system uses optical magnification and a high-resolution camera to image particles as they stream through the flow cell. As the particle flow, each particle is imaged separately enabling the acquisition of hundreds to thousands of particles per second. Each particle image is then automatically analyzed to 50 different shape/color/fluorescence parameters that are used to classify and quantify the particle populations.

The Flowsight© is the ‘little brother’ of the ImagestreamX, combining the speed of flow cytometry and resolution of microscopy. It contains a single 20x maginifcation lens, best-suited for cell-wide studies rather than focussing on internal probes. It can image and quantify signals from up to 12 fluorescent probes and 5 lasers, at a resolution of 1-2μm2. Despite its internal complexity, the program is extremely easy-to-use with guided “wizards” or stream-lined workflows, enabling the user to get the most data possible out of his/her sample.