Flow Cytometry (FACS) Analyzers

Overview

In recent years, there is a wide offering of flow cytometry analyzers from numerous manufacturers. As a researcher, trying to ascertain which of these flow cytometers is optimal for your own research needs is a daunting undertaking. With almost 4 decades of experience in the field of flow cytometry, we will be happy to provide you with a no-obligation analysis of your flow cytometry needs – and advise the pros and cons of any flow cytometers you are considering to purchase. Please feel free to call us to discuss this further.

Use Cases

• Clinical Diagnostics: Leukemia/lymphoma classification, HIV monitoring, and transplant compatibility testing where results directly impact patient care

• Immunology Research: Phenotyping complex immune populations where subtle shifts in cell subsets drive disease

• Drug Development: Mechanism of action studies where understanding which specific cells respond to your compound can save millions in development costs

• Microbiology: Rapidly assessing microbial viability or antibiotic resistance where time matters

• Stem Cell Research: Purifying and characterizing stem cell populations where cellular heterogeneity is the norm

Unique Benefits

• Single-cell detail AND population statistics simultaneously. This isn't just nice to have - it's the difference between seeing that your cancer drug kills 30% of "cells" versus discovering it kills 90% of tumor cells but none of the contaminating fibroblasts.

• The multi-parameter capability lets you see relationships that are completely invisible otherwise. I've watched researchers have "eureka" moments discovering cell populations they'd missed for years because they couldn't correlate 5+ markers simultaneously before.

• For rare events, FACS is often the only practical option. When you're looking for circulating tumor cells at 1-in-10,000 frequency, microscopy becomes an exercise in futility while FACS just works.

• You're analyzing intact, viable cells that can be sorted and used in downstream applications - not just cellular debris after lysis.

• When you need to understand complex cellular heterogeneity, there's often no substitute for flow cytometry.

The ID7000 spectral flow cytometer from Sony Biotechnology revolutionizes the analysis of complex cell populations. Unlike traditional flow cytometers with pre-defined fluorescence channels, the ID7000 leverages cutting-edge spectral detection technology. This approach captures the complete emission spectrum for each fluorescent signal, enabling unparalleled resolution and differentiation of even overlapping fluorochromes. The system boasts industry-leading capabilities with the ability to accommodate up to 8 lasers and run fluorescent panels exceeding 40 colors simultaneously. This translates into an exceptional level of multiplexing, allowing researchers to explore a vast array of cellular markers in a single experiment.

Stratedigm builds compact, flexible flow cytometers and an automation suite designed around them. By controlling every step of high-throughput cytometry — plate handling, acquisition and analysis — it lets a laboratory set up multi-plate experiments and work through thousands of samples in one software environment, from single plates and Eppendorf tubes up to hundreds of plates.

Flow Cytometry is an extremely effective tool for cell counting and molecular analysis but is usually limited to particles larger than ~400nm. Many emerging fields such as virology, microbiology, extra-cellular particles, and exosomes require higher sensitivity. The Apogee A60’s novel optical design allows detection of particles from 110nm thus making FACS analysis available for these disciplines. With up to 3 forward scatter detectors and 9 fluorescent channels, it is now possible to classify small particles at great speed and with robust statistics.

Flow Cytometry (FACS) Analyzers

Sony ID7000 Spectral Cytometry

Stratedigm Flow Cytometry Automation

Apogee A60 Micro