Microscopy

Overview
Intravital, Two Photon and Unlabeled Live Microscopy provide exquisite high quality cell images, which may subsequently be analyzed using unique software – to provide both qualitative and quantitative data on the tissue and cells being imaged.
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Use Cases
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Unique Benefits
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Circulating Tumor Cells (CTC) in Clinical Research: CTC Enumeration

RareCyte enables practical deployment of circulating tumor cell (CTC) and rare cell-based liquid biopsy with instrumentation and consumables that provide an exquisitely sensitive, accurate, reproducible, and transparent workflow from blood collection to single cell isolation.

  • Unbiased, reproducible, highly sensitive, and specific rare cell detection
  • Scalable, high-capacity platform with workflow breakpoints, including slide banking, that enable multi-site execution
  • Deploy through our global contract research organization (CRO) network or in your own lab
  • Utilize cancer-specific panels or Developer panels for CTC biomarker analysis
  • Custom assay and companion diagnostics co-development through our Precision Biology Services

Publication: Circulating tumor cell detection: A prospective comparison between CellSearch® and RareCyte® platforms in patients with progressive metastatic breast cancer

https://link.springer.com/article/10.1007/s10549-022-06585-5

 

High Sensitivity for Precise Single-Cell Analysis and Retrieval

  • Sensitivity: Platform tailored for ultra-rare cell collection, identification, and isolation
  • Specificity: Protein expression heterogeneity among rare target cells revealed with up to six channels, and image-based cell identification and selection ensures integrity of single cell data
  • Retrieval: Gentle single cell isolation from multiple sample preparation types that enable both DNA and RNA analysis

CTC surrounded by white blood cell

SK-BR-3 model CTC surrounded by white blood cells on AccuCyte® slide. CK / EpCAM (magenta) / nuclei (blue).
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Enable whole slide High Plex tissue analysis for Research and Clinical Applications

Orion is a novel technology and services platform offering the fastest path to

whole slide, high-plex imaging. Combining speed and subcellular resolution,

Orion enables comprehensive phenotypic profiling and characterization of

tissue architecture, tumor heterogeneity, and the immune response for whole

slide tissue sections in hours, not days.

Exceptional signal-to-noise ratio provides robust quantification of biomarkers

across a broad dynamic range. The ability to measure changes in expression of

low-level markers supports translational and clinical research.

Run panels from an extensive biomarker list, add in custom biomarkers, or

engage our services lab for custom panels and programs.

Orion instrument specifications

  • Full-slide scan in 75 minutes per cm2 at 20x
  • Flexible panel design – use your own antibodies
  • Up to 16 Plex biomarker panels
  • Same slide immunofluorescent and H&E imaging
  • Use standard slides and sample preparation
  • Easily fits on a standard laboratory benchtop

For comprehensive specifications, download the Orion specification sheet.

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Nanolive’s imaging and analysis platform – the CX-A – is the only solution in the world to offer automated 3D label-free live cell imaging that is compatible with 96-well plates.

* Non-invasive autofocus solution: keeps cells in focus for the time of the experiment.

* Integrated image analysis solution with multi-parametric read-out.

* Long-term imaging of living cells with no perturbations and no contamination in physiological conditions thanks to our dedicated incubation solution. More details here.

* High quality motorized stage with a micron range precision repeatability of motion.

* Stitching feature: allows to analyze highly confluent cell populations containing hundreds of cells while keeping Nanolive’s signature sub-cellular resolution.

* Meaningful correlative imaging: features a fully integrated 3 channel epifluorescence imaging modality.

 

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Intravital microscopy enables dynamic 3D cellular-level imaging of various biological processes in living animals in vivo. It enables scientists to directly verify hypotheses derived from ex vivo or in vitro observations in natural physiological in vivo microenvironments.
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