Nanoparticles FACS
Nanoparticles have been under increasing investigation in many different fields (medicine, food industry, environmental science, etc) due to their potential in a variety of applications. These applications range from determining nanomedicine effectiveness, engineered nanomaterial safety assurance to quantifying nanoparticle uptake by cells. Given the substance specific dependence of cellular uptake on size, shape, surface charge, […]
NFkB
NFkB is a transcription factor involved in the regulation of genes encoding mediators of the immune, acute phase and inflammatory responses. The transcription factor binds to a specific DNA sequence (5´ - GGGACTTTCC - 3´) in the promoter region of these genes. The protein complex which makes up NFkB is composed of Rel family homo […]
IBD imaging
Inflammatory bowel disease (IBD) is a chronic idiopathic disease in the gastrointestinal (GI) tract. It encompasses two interrelated intestinal disorders; Crohn’s disease (CD) and ulcerative colitis (UC). It is generally accepted that IBD results from an excessive immune reaction to to gut luminal microbes. It occurs in genetically susceptible individuals but is triggered by exposure […]
Zeta potential
Zeta potential is characterization technique that can be used to determine the surface charge of nanoparticles in solutions – something that is obviously of great importance when developing new drug delivery carriers. What happens is that the surface of the charged particle attracts molecules with opposite charges and binds to them. This generates a thin […]
Cell sorting
If you would have told cell biologists two generations ago that their intellectual descendants would be able to place trypsinized cells from an in-vitro cell culture or in-vivo tissue samples, and physically separate and collect user-defined cell populations with a specialized sorter sort out specific subpopulations for further experimentation and manipulation, they would have been […]
Extracellular Vesicles
Extracellular Vesicles such as Exosomes are small membrane vesicles (30–150 nm) of endocytic origin, All cells secret them constantly under both healthy and pathological conditions and they can be found in various body fluids including blood, saliva, urine, semen, bile, ascites, and gastrointestinal lavage fluid. Exosomes can contain proteins, nucleic acids (DNA fragments as well as […]
Biomarkers
Biomarkers, coined from an almagm of biological and marker, refer to a vast range of chemicals and physiological phenomena. Defined by the NIH as “a characteristic that is objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention.” it can be used for diagnosis, prognosis, […]
Digital Elisa
What is it ELISA? Enzyme-linked immune absorbent assay or ELISA is the tried and true, quick and dirty way of assessing the immune response of the body to vaccines, infections or other immune challenges. It has other applications as well, however. The food industry typically uses ELISA to detect the presence of allergens in food […]
Non-Destructive Testing: Improving Research and Quality Control
What is Non-Destructive Testing? Nobody likes to waste time, not in the field of construction and not in other additional fields. No one likes to find himself going through a series of bureaucratic procedures that not only do not bring benefit but bring mostly harm. But, naturally, we cannot create anything in the industrial world, […]
Mice imaging
Mouse imaging Mice are valuable laboratory animals, with a long history of use in medical research. However, the use of mice in biomedical research has come under increasing criticism in recent years. The reasons include the high mortality of rodents used in laboratories and the misuse of data derived from these studies. Today's more sophisticated […]